【储存条件】
长期保存,请置于4˚C (切勿结冰冻住)。
【产品简介】
M5 Hiper Lipo2500 Transfection Reagent is a proprietary formulation for transfecting nucleic acids into a wide range of eukaryotic
cells. DNA-Lipo 2500 complexes must be made in serum-free medium such as Opti-MEM® Reduced Serum Medium and can be added directly to cells in culture medium, in the presence or absence of serum/antibiotic. It is not necessary to remove complexes or change/add medium after transfection. The amount of Lipo 2500 Reagent required for successful transfection varies depending on the cell type and passage number. Start any new transfection by testing the recommended four concentrations of Lipo2500 Reagent to determine an optimum amount.
【产品组分】
MF135-plus-01 MF135-plus-02
M5 Hiper Lipo2500 Transfection Reagent 0.75 ml 1.5 ml
【实验前准备】
1. Plate cells so they will be 70–90% confluent at the time of transfection.
2. Prepare plasmid DNA-lipid complexes.
3. Add DNA-lipid complexes to cells.
【操作步骤】
A、Lipo2500 DNA Transfection Reagent Protocol(具体方法见后面)
Component 96-well 24-well 6-well
Final DNA per well 100 ng 500 ng 2500 ng
Final Lipo2500 Reagent per well 0.2–0.5 μL 1.0–2.5 μL 5.0–12.5 μL
B、Co-Transfection of Plasmid DNA and siRNA
Transfect plasmid DNA and siRNA at the same time using Lipo2500 Reagent by adding 30 pmol (~0.6 μg) of siRNA per 1 μg of DNA.
C、mRNA Transfection
mRNA can be transfected in a 24-well plate using Lipo2500 Reagent by adding 0.5–1 μg of mRNA per well.
D、Lipo 2500 DNA Transfection Reagent Protocol(具体方法)
Transfect cells according to the following chart. Volumes are given on a per-well basis. Each reaction mix is sufficient for triplicate (96-well), duplicate (24-well), and single well (6-well) transfections, and accounts for pipetting variations. Adjust the amounts of components according to your tissue culture format. For additional information on scaling your transfection reaction, see page 1.
(Lip2500使用方法和Lip2000完全一样,但是效率更高,对细胞毒性更小)
- 正品保障
高额赔付 - 8000万+
试剂耗材仪器 - 期货精准
现货次日到达 - 便捷价优
省钱省时省心
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